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KMID : 0380020050200010034
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 1 p.34 ~ p.39
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Optimization of Culture Conditions for the 【+】-Eudesmin Production In Magnolia sieboldii Cells
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Hwang Sung-Jin
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Abstract
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In order to product the furofuranoid lignans, (+)-eudesmin which is one of the secondary products from Magnolia sieboldii. through cell suspension cultures; various culture media, initial sucrose concentration, elicitations, shaking speeds, and inoculum sizes. Among the culture media tested, MS medium had a pronounced effect on suspension cell growth and (+)-eudesmin contents. The maximum dry cell weight (DCW) of 3.71 g per flask was obtained at inoculum size of 0.5 g and in MS medium supplemented with sucrose plus 0.5 mg/L 2,4-D after 8 weeks. (+)-Eudesmin biosynthesis was stimulated with high initial sucrose concentration ,and the maximum (+)-eudesmin production of DCW was achieved at 200mg/L chitosan and initial medium sucrose. The optimal shaking speeds for dry biomass accumulation and (+)-eudesmin contents was 130 rpm. This work is considered to be helpful for large-scale bioprocessing of Magnolia sieboldii suspension cell cultures in bioreactor.
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KEYWORD
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Magnolia sieboldii, suspension cell cultures, (+)-eudesmin
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